TOSOH Product

AFC(Affinity Chromatography)


Affinity Chromatography

Affinity chromatography (AFC) is performed on a support that is functionalized with a ligand that shows biological affinity for a particular enzyme or other biological molecule.  

Almost all biomolecules can be purified on the basis of specific interaction between their chemical or biological structure and a suitable affinity ligand. Typical molecular pairs are antigens and antibodies, enzymes and coenzymes, and sugars with lectins. Thus, affinity chromatography distinguishes itself from, e.g., reversed phase and ion exchange chromatography, in that a highly specific interaction with the protein of interest is the cause for separation or purification. 

Although affinity chromatography is not specific, in that no enzyme interacts with only one substrate, it is the most selective method for separating proteins. The selectivity is often based on spatial recognition via a 'lock-and-key' mechanism, as in antibody-antigen interactions. 

Less selective general ligands have affinity for a whole class of proteins, which often require other chromatographic methods for purification of individual members. The term bioselective adsorption has been proposed as an alternative name for affinity chromatography.

Affinity Chromatography (AFC) offers the greatest potential specificity and selectivity for the isolation or purification of biomolecules.  To enhance this advantage, three group specific ligands and one chemically active functionality are available through Tosoh Bioscience.

All analytical TSKgel AFC columns are based on the well-established 10 micron rigid TSKgel G5000PW resin. This resin features 100 nm pores that have an estimated exclusion limit of < 10 million Da along with excellent stability from pH 2 to 9.